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KMID : 1007520160250051393
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Food Science and Biotechnology
2016 Volume.25 No. 5 p.1393 ~ p.1398
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Strategies in fed-batch cultivation on the production performance of Lactobacillus salivarius I 24 viable cells
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Ming Lim Chi
Halim Murni
Rahim Raha Abd
Wan Ho Yin
Ariff Arbakariya Bin
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Abstract
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The potential use of fed-batch cultivation (FBC) for improvement of the production of Lactobacillus salivarius I 24 biomass for subsequent use as probiotics was studied using a 2-L stirred-tank bioreactor. Three different constant feeding rates (0.1, 0.05, and 0.033 L/h) were applied in FBCs and their effect on carbon metabolism was evaluated. The carbon flux for cell built-up with reduction in lactic acid synthesis was observed in the fed-batch as compared to the batch cultivation mode. The viable cell number obtained in the constant FBC (CFBC) operated at a feeding rate of 0.05 L/h was 8 times higher (10.7×1010 CFU/mL) than that recorded in the batch cultivation. This gave the viable cell yield based on glucose consumed for CFBC of 26 times higher (11.3×1012 CFU/gGlucose) than the batch cultivation. This study demonstrated CFBC, which is simple with minimal use of process control equipment, has an industrial potential for improvement of probiotic production.
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KEYWORD
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fed-batch cultivation, Lactobacillus salivarius, lactic acid bacteria, probiotics, carbon metabolism
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